Decrease in mitochondrial energy coupling by thyroid hormones: a physiological effect rather than a pathological hyperthyroidism consequence

• Published in: FEBS letters Vol. 430; no. 3; pp. 409 - 413
• Main Authors: Bobyleva, V, Pazienza, T.L, Maseroli, R, Tomasi, A, Salvioli, S, Cossarizza, A, Franceschi, C, Skulachev, V.P
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 03.07.1998
• Subjects: Animals; Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; Energy Metabolism - physiology; FCCP, p-trifluoro-methoxycarbonylcyanide phenylhydrazone; Hepatocyte; Hyperthyroidism - chemically induced; Hyperthyroidism - metabolism; Hypothyroidism - chemically induced; Hypothyroidism - metabolism; JC-1, 5,5′,6,6′-tetrachloro-1,1′3,3′-tetraethylbenzimidazolylcarbocyan iodine; kCh, 6-ketocholestanol (5α-cholestan-3β-ol-6-one); Ketocholesterols - pharmacology; Liver - metabolism; Male; Membrane potential; Membrane Potentials; Mitochondria, Liver - metabolism; Mitochondria, Liver - physiology; Mitochondrion; Oxygen Consumption; Rats; Rats, Sprague-Dawley; T3, 3,5,3′-triiodothyronine; Thyroid hormone; Thyroid Hormones - physiology; UCP, uncoupling protein; Uncoupling; Uncoupling Agents; ΔΨm, mitochondrial transmembrane electric potential difference; Mitochondrion; Uncoupling; ΔΨ m, mitochondrial transmembrane electric potential difference; FCCP, p-trifluoro-methoxycarbonylcyanide phenylhydrazone; T 3, 3,5,3′-triiodothyronine; kCh, 6-ketocholestanol (5α-cholestan-3β-ol-6-one); Hepatocyte; UCP, uncoupling protein; Thyroid hormone; Membrane potential; JC-1, 5,5′,6,6′-tetrachloro-1,1′3,3′-tetraethylbenzimidazolylcarbocyan iodine
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/S0014-5793(98)00700-5

The effect of the in vivo thyroid status on mitochondrial membrane potential (ΔΨ m) in isolated rat hepatocytes was studies by means of a cytofluorimetric technique and the ΔΨ m-specific probe JC-1. It is shown that the ΔΨ m level decreases in the order hypothyroid>euthyroid>hyperthyroid. Polarographic measurement of the hepatocyte respiratory rates revealed an opposite trend of values: the highest respiratory rate in hepatocytes from hyperthyroid animals, the lowest in those from hypothyroid ones. This means that mitochondrial energy coupling is highest in hypothyroid hepatocytes and lowest in hyperthyroid hepatocytes. 6-Ketocholestanol added to hepatocytes failed to counterbalance the uncoupling effect of thyroid hormones on ΔΨ m and respiration rate. Under the same conditions, 6-ketocholestanol appeared to be effective in recoupling of respiration uncoupled by low concentrations of the artificial protonophore FCCP. The mechanism and possible physiological functions of the thyroid hormone-induced decrease in mitochondrial energy coupling are discussed.