Angiotensin II restricted analogs with biological activity in the erythrocytic cycle of Plasmodium falciparum

• Published in: Journal of peptide science Vol. 21; no. 1; pp. 24 - 28
• Main Authors: Torres, Marcelo Der Torossian, Silva, Adriana Farias, de Souza Silva, Leandro, de Sá Pinheiro, Ana Acácia, Oliveira, Vani Xavier Jr
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.01.2015; Wiley Subscription Services, Inc
• Subjects: Angiotensin II - analogs & derivatives; Angiotensin II - pharmacology; Antimalarials - pharmacology; Cells, Cultured; Erythrocytes - parasitology; Humans; malaria; Peptide Fragments; Peptides; Plasmodium falciparum; Plasmodium falciparum - drug effects; Plasmodium gallinaceum; restriction; sporozoites; restriction; Plasmodium falciparum; peptides; malaria; sporozoites
• ISSN: 1075-2617; 1099-1387
• DOI: 10.1002/psc.2714

The anti‐plasmodial activity of conformationally restricted analogs of angiotensin II against Plasmodium gallinaceum has been described. To observe activity against another Plasmodium species, invasion of red blood cells by Plasmodium falciparum was analyzed. Analogs restricted with lactam or disulfide bridges were synthesized to determine their effects and constraints in the peptide–parasite interaction. The analogs were synthesized using tert‐butoxycarbonyl and fluoromethoxycarbonyl solid phase methods, purified by liquid chromatography, and characterized by mass spectrometry. Results indicated that the lactam bridge restricted analogs 1 (Glu‐Asp‐Arg‐Orn‐Val‐Tyr‐Ile‐His‐Pro‐Phe) and 3 (Asp‐Glu‐Arg‐Val‐Orn‐Tyr‐Ile‐His‐Pro‐Phe) showed activity toward inhibition of ring formation stage of P. falciparum erythrocytic cycle, preventing invasion in about 40% of the erythrocytes. The disulfide‐bridged analog 10 (Cys‐Asp‐Arg‐Cys‐Val‐Tyr‐Ile‐His‐Pro‐Phe) was less effective yet significant, showing a 25% decrease in infection of new erythrocytes. In all cases, the peptides presented no pressor activity, and hydrophobic interactions between the aromatic and alkyl amino acid side chains were preserved, a factor proven important in efficacy against P. gallinaceum. In contrast, hydrophilic interactions between the Asp1 carboxyl and Arg2 guanidyl groups proved not to be as important as they were in the case of P. gallinaceum, while interactions between the Arg2 guanidyl and Tyr4 hydroxyl groups were not important in either case. The β‐turn conformation was predominant in all of the active peptides, proving importance in anti‐plasmodial activity. This approach provides insight for understanding the importance of each amino acid residue on the native angiotensin II structure and a new direction for the design of potential chemotherapeutic agents. Copyright © 2014 European Peptide Society and John Wiley & Sons, Ltd. N‐terminal restricted angiotensin II analogs presented anti‐plasmodial activity against Plasmodium falciparum.Active lactam bridged analogs presented higher activity than disulphide bridged ones.i‐(i+2) restrictions showed to be more active depending on the bridgehead elements.The β‐turn conformation was predominant in all of the active peptides, proving importance in anti‐plasmodial activity.