Optimization and validation of CEDIA drugs of abuse immunoassay tests in serum and urine on an Olympus AU 400

A preliminary initial cloned enzyme donor immunoassay (CEDIA) was optimized for serum and urine drug testing with respect to the German per se limits for driving under the influence of drugs (serum) and lowered cut-offs in cases of driving licence re-granting (urine). The tests were performed on an...

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Bibliographic Details
Published inDrug testing and analysis Vol. 5; no. 5; p. 366
Main Authors Musshoff, F, Wolters, T, Lott, S, Ippisch, J, Gradl, S, Madea, B
Format Journal Article
LanguageEnglish
Published England 01.05.2013
Subjects
Automobile Driving
Humans
Immunoenzyme Techniques - methods
Sensitivity and Specificity
Street Drugs - blood
Street Drugs - urine
Substance Abuse Detection - methods
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Summary:A preliminary initial cloned enzyme donor immunoassay (CEDIA) was optimized for serum and urine drug testing with respect to the German per se limits for driving under the influence of drugs (serum) and lowered cut-offs in cases of driving licence re-granting (urine). The tests were performed on an Olympus AU 400 auto analyzer. Validation revealed sensitivities between 93% and 100% based on comparison with data from gas or liquid chromatography coupled with mass spectrometry. Even if specificity ranged between 83% and 98 %, the tests can be considered useful for forensic purposes. Receiver operating characteristic (ROC) curves, Youden indices, as well as positive and negative predictive values are presented.
ISSN:1942-7611
DOI:10.1002/dta.1454