The Aberrant Expressions of Nuclear Matrix Proteins During the Apoptosis of Human Osteosarcoma Cells

• Published in: Anatomical record (Hoboken, N.J. : 2007) Vol. 293; no. 5; pp. 813 - 820
• Main Authors: Zhao, Zhen‐Li, Li, Qi‐Fu, Zheng, Yan‐Bin, Chen, Lan‐Ying, Shi, Song‐Lin, Jing, Guang‐Jun
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.05.2010
• Subjects: Antineoplastic Agents - toxicity; Apoptosis; Apoptosis - drug effects; Apoptosis - genetics; Blotting, Western; Bone diseases; Bone Neoplasms - genetics; Bone Neoplasms - metabolism; Bone Neoplasms - physiopathology; Cell cycle; Cell Line, Tumor; cell morphology; Cell Nucleus - drug effects; Cell Nucleus - pathology; Chromatin; Chromatin - drug effects; Chromatin - pathology; Curcumin; Curcumin - toxicity; Electrophoresis, Gel, Two-Dimensional; Flow Cytometry; Gel electrophoresis; Gene expression; Gene Expression Regulation, Neoplastic - drug effects; Gene Expression Regulation, Neoplastic - genetics; human osteosarcoma cell; Humans; Immunoblotting; matrix protein; Microscopy, Electron, Transmission; nuclear matrix protein; Nuclear Matrix-Associated Proteins - drug effects; Nuclear Matrix-Associated Proteins - genetics; Nuclear Matrix-Associated Proteins - metabolism; Nuclear Proteins - drug effects; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Nuclei; Osteosarcoma; Osteosarcoma - genetics; Osteosarcoma - metabolism; Osteosarcoma - physiopathology; Osteosarcoma cells; prohibitin; Repressor Proteins - drug effects; Repressor Proteins - genetics; Repressor Proteins - metabolism; Transmission electron microscopy; Vimentin; Vimentin - drug effects; Vimentin - genetics; Vimentin - metabolism; Western blotting
• ISSN: 1932-8486; 1932-8494; 1932-8494
• DOI: 10.1002/ar.21074

The objective of this study was to investigate altered expressions of nuclear matrix proteins (NMPs) of human osteosarcoma (OS) MG‐63 cells during curcumin‐induced apoptosis of human OS MG‐63 cells. MG‐63 cells were cultured with curcumin (7.5 mg/L) for 72 hr. Morphological alterations of cells were captured using light microscopy and transmission electron microscopy, and cell cycle distribution was estimated by flow cytometry. NMPs were selectively extracted and subjected to two‐dimensional gel electrophoresis (2‐DE) analysis. Western blots were performed to determine changes in the expression levels of specific NMPs. The results demonstrated that typical characteristics of apoptosis were observed. Cellular chromatin agglutinated, cell nuclei condensed, and apoptotic bodies were formed after treatment with curcumin. The 2‐DE results displayed 27 NMPs, 21 of which were identified to have change in expression levels significantly during apoptosis. The altered expressions of three of these NMPs (nucleophosmin, prohibitin, and vimentin) were further confirmed by immunoblotting. These findings indicated that the apoptosis of MG‐63 cells was accompanied by the expression alteration of NMPs. Our results might help to reveal the relationship between NMPs and the regulation of gene expression in the process of apoptosis, as well as provide the basic concepts for future studies on the mechanisms of apoptosis and the therapy for bone diseases. Anat Rec, 2010. © 2010 Wiley‐Liss, Inc.