Encapsulation of interleukin-2 in murine erythrocytes and subsequent deposition in mice receiving a subcutaneous injection

Radiolabeled recombinant human interleukin-2 (IL-2) was successfully encapsulated in both mouse and sheep erythrocytes. Of the added IL-2, 70% was recovered bound to or encapsulated within the carrier cells. Erythrocytes containing IL-2 were stable in vitro and most of the IL-2 remained associated w...

Full description

Saved in:
Bibliographic Details
Published inBiotechnology and applied biochemistry Vol. 10; no. 2; p. 183
Main Authors DeLoach, J.R, Andrews, K, Sheffield, C.L
Format Journal Article
LanguageEnglish
Published United States 01.04.1988
Subjects
Animals
ERITROCITOS
ERYTHROCYTE
ERYTHROCYTES
Humans
IMMUNE RESPONSE
INJECTION
Injections, Subcutaneous
Interleukin-2 - administration & dosage
Interleukin-2 - pharmacokinetics
INYECCION
LINFOCITOS
LYMPHOCYTE
LYMPHOCYTES
MICE
Mice, Inbred BALB C
OVIN
OVINOS
RATON
Recombinant Proteins - administration & dosage
Recombinant Proteins - pharmacokinetics
REPONSE IMMUNITAIRE
RESPUESTA INMUNOLOGICA
SHEEP
SOURIS
Sulfur Radioisotopes
Tissue Distribution
Online AccessGet more information

Cover

More Information
Summary:Radiolabeled recombinant human interleukin-2 (IL-2) was successfully encapsulated in both mouse and sheep erythrocytes. Of the added IL-2, 70% was recovered bound to or encapsulated within the carrier cells. Erythrocytes containing IL-2 were stable in vitro and most of the IL-2 remained associated with the cells following a 16-h incubation at 37 degrees C. When carrier erythrocytes containing IL-2 were injected subcutaneously into mice, intact [35S]IL-2 was detectable in a number of tissues 3 days after injection.
Bibliography:L50
8915292
ISSN:0885-4513
1470-8744
DOI:10.1111/j.1470-8744.1988.tb00013.x