Antioxidant properties and xanthine oxidase inhibitory effects of Tamus communis L. root extracts

• Published in: Phytotherapy research Vol. 23; no. 2; pp. 283 - 288
• Main Authors: Boumerfeg, Sabah, Baghiani, Abderrahmane, Messaoudi, Dalila, Khennouf, Seddik, Arrar, Lekhmici
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.02.2009; Wiley
• Subjects: Algeria; Animals; antioxidant; Antioxidants - pharmacology; Biological and medical sciences; Cattle; Dose-Response Relationship, Drug; Flavonoids - isolation & purification; Free Radical Scavengers - pharmacology; General pharmacology; Humans; Medical sciences; medicinal plant; Medicine, Traditional; Milk - enzymology; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phenols - isolation & purification; Plant Extracts - pharmacology; Plant Roots - chemistry; Polyphenols; Sheep; superoxide scavenger; Tamus - chemistry; TRAP; xanthine oxidase; Xanthine Oxidase - antagonists & inhibitors; Algeria; Root; Enzyme; Pharmacognosy; Extract; TRAP; Antioxidant; Radical scavenger; Medicinal plant; Hyperoxides; Plant origin; Xanthine oxidase; Oxidoreductases; superoxide scavenger; Below ground plant part
• ISSN: 0951-418X; 1099-1573
• DOI: 10.1002/ptr.2621

This study was conducted to search for xanthine oxidase (XO) inhibitors from the root extracts of Tamus communis L. traditionally used in folk medicine in Algeria. Root extracts with different solvents were screened for purified milk xanthine oxidase inhibition. The root extracts (methanol, chloroform and ethyl acetate) and proteins, obtained in distilled water, inhibited bovine, sheep and human milk XO from three species in a concentration‐dependent manner, with an additional superoxide scavenging capacity, which reached its highest level with ethyl acetate extract (IC50 = 0.15, 0.04 and 0.09 g/L) for bovine XO, sheep XO and human XO, respectively. The antioxidant potential was confirmed with the non‐enzymatic method, total radical‐trapping antioxidant parameter (TRAP) assay, which showed that the Tamus communis L. extracts have a potential antioxidant activity in the same order obtained by using the reduction of cytochrome c, an enzymatic method, in which the antioxidant activity followed a decreasing order: ethyl acetate extract > chloroform extract > protein. Copyright © 2008 John Wiley & Sons, Ltd.