Ferrous Chelator 2,2′-Dipyridyl Attenuates Cerebral Vasospasm after Experimental Subarachnoid Haemorrhage in Rabbits

• Published in: Journal of international medical research Vol. 38; no. 2; pp. 583 - 592
• Main Authors: Yu, YY, Niu, L, Gao, L, Zhang, GL, Li, J, Deng, JP, Qu, YZ, Zhao, ZW, Gao, GD
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.03.2010
• Subjects: 2,2'-Dipyridyl - pharmacology; Animals; Apoptosis - drug effects; Blotting, Western; Chelating Agents - pharmacology; Disease Models, Animal; Immunoblotting; Immunoenzyme Techniques; Male; Rabbits; Subarachnoid Hemorrhage - drug therapy; Tumor Suppressor Protein p53 - metabolism; Vasospasm, Intracranial - prevention & control; Cerebral vasospasm; Subarachnoid haemorrhage; 2,2′-Dipyridyl; Apoptosis; p53
• ISSN: 0300-0605; 1473-2300
• DOI: 10.1177/147323001003800220

The effect of 2,2′-dipyridyl (DP) on cerebral vasospasm was investigated in a double-injection rabbit model of subarachnoid haemorrhage (SAH). Thirty-six animals were divided between four groups: control (sham-operated), SAH (model alone), SAH + DP (the SAH model in which DP dissolved in dimethyl sulphoxide [DMSO] was injected once daily for 5 days into the cisterna magna), and SAH + DMSO (the SAH model in which DMSO [vehicle] was injected daily for 5 days). There were significant differences in the basilar artery luminal area, wall thickness, neurological deficit score and vasospasm index between the SAH + DP and SAH groups. There was a significant negative correlation between arterial luminal area and arterial wall thickness, and also between the neurological deficit score and vasospasm index. Cells that were positive for terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end-labelling (TUNEL) and p53 expression were significantly increased in the SAH + DMSO and SAH groups, but not in the SAH + DP group, versus controls. Thus, DP may attenuate cerebral vasospasm after SAH by suppressing p53-induced apoptosis in the cerebral vessels.