Expression of a novel function-associated molecule on cells mediating spontaneous cytolysis in swine

• Published in: Developmental and comparative immunology Vol. 17; no. 3; pp. 277 - 282
• Main Authors: Camenisch, Todd D., Jaso-Friedmann, Liliana, Evans, Donald L., Harris, David T.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.05.1993; Elsevier Science
• Subjects: Animals; Antibodies, Monoclonal; Biological and medical sciences; Biological Factors - immunology; Biological Factors - metabolism; Cells, Cultured; Cytotoxicity, Immunologic; Fishes - immunology; Function-associated molecule; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Immunobiology; In Vitro Techniques; Killer Cells, Natural - immunology; Lymphocytes - immunology; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Natural killer; Nonspecific immunity; Spontaneous cytotoxicity; Swine - immunology; Swine lymphocytes; Swine lymphocytes; Function-associated molecule; Spontaneous cytotoxicity; Natural killer; Nonspecific immunity; Cell culture; Biological evolution; Cytotoxicity; Monoclonal antibody; Cell surface; Pig; Natural killer cell; Proteins; Vertebrata; Mammalia; LAK cell; Artiodactyla; Detection; Ungulata
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/0145-305X(93)90046-S

In the present study, we have analyzed the effects of the anti-FAM mAb 5C6, on endogenous NK cell and lymphokineactivated killer (LAK) cell activity of swine. We report that the anti-FAM mAb 5C6 binds to fresh and cultured lymphocytes with spontaneous cytotoxic activity from swine peripheral blood. In conjunction, the anti-FAM mAb 5C6 inhibits swine cytolysis against K562 target cells. Inhibition was found to be mediated by blocking of conjugate formation. The level of spontaneous killing and mAb inhibition was correlated with the number of swine cells staining positive with the mAb 5C6 as assessed by flow cytometry.