A sensitive electrochemical biosensor for detection of DNA methyltransferase activity by combining DNA methylation-sensitive cleavage and terminal transferase-mediated extension

A novel electrochemical biosensor was developed for activity assay of DNA methyltransferase and its inhibitor based on methylation-sensitive cleavage, which activated a primer for terminal transferase-mediated extension of biotinylated dUTP followed by sensitive detection via enzymatic amplification...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 48; no. 50; pp. 6280 - 6282
Main Authors Wu, Huiwang, Liu, Shucheng, Jiang, Jianhui, Shen, Guoli, Yu, Ruqin
Format Journal Article
LanguageEnglish
Published England 01.01.2012
Subjects
Biosensing Techniques
DNA Cleavage - drug effects
DNA Methylation - drug effects
DNA Modification Methylases - antagonists & inhibitors
DNA Modification Methylases - metabolism
Electrochemical Techniques
Enzyme Activation
Enzyme Inhibitors - pharmacology
Structure-Activity Relationship
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Summary:A novel electrochemical biosensor was developed for activity assay of DNA methyltransferase and its inhibitor based on methylation-sensitive cleavage, which activated a primer for terminal transferase-mediated extension of biotinylated dUTP followed by sensitive detection via enzymatic amplification.
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ISSN:1359-7345
1364-548X
DOI:10.1039/c2cc32397d