Absence of normal keratan sulfate in the blood of patients with macular corneal dystrophy

We measured levels of sulfated keratan sulfate in serum using a monoclonal antibody in an enzyme-linked immunosorbent assay. Sulfated keratan sulfate was not detected in the serum of 16 patients with macular corneal dystrophy, but was present at normal levels in 66 patients with other corneal diseas...

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Bibliographic Details
Published inAmerican journal of ophthalmology Vol. 102; no. 5; p. 561
Main Authors Thonar, E J, Meyer, R F, Dennis, R F, Lenz, M E, Maldonado, B, Hassell, J R, Hewitt, A T, Stark, Jr, W J, Stock, E L, Kuettner, K E
Format Journal Article
LanguageEnglish
Published United States 15.11.1986
Subjects
Adolescent
Adult
Aged
Antibodies, Monoclonal
Antibody Specificity
Corneal Dystrophies, Hereditary - blood
Female
Glycosaminoglycans - blood
Humans
Keratan Sulfate - blood
Male
Middle Aged
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Summary:We measured levels of sulfated keratan sulfate in serum using a monoclonal antibody in an enzyme-linked immunosorbent assay. Sulfated keratan sulfate was not detected in the serum of 16 patients with macular corneal dystrophy, but was present at normal levels in 66 patients with other corneal diseases. There were no differences with respect to age, sex, and other ocular findings. This monoclonal antibody recognizes a sulfated carbohydrate epitope present in both corneal and skeletal keratan sulfate. Since most serum keratan sulfate is derived from the cartilages, the defect in keratan sulfate synthesis in macular corneal dystrophy may not be restricted to corneal cells. This assay should prove useful in the diagnosis of macular corneal dystrophy, particularly in children at risk before the appearance of opacification.
ISSN:0002-9394
1879-1891
DOI:10.1016/0002-9394(86)90525-8