A new turn-on fluorescent probe for selective detection of glutathione and cysteine in living cells

• Published in: Chemical communications (Cambridge, England) Vol. 49; no. 41; pp. 4640 - 4642
• Main Authors: Wei, Mingjie, Yin, Peng, Shen, Youming, Zhang, Lingli, Deng, Jianhui, Xue, Shanyan, Li, Haitao, Guo, Bin, Zhang, Youyu, Yao, Shouzhuo
• Format: Journal Article
• Language: English
• Published: England 01.01.2013
• Subjects: chemical communication; chemical reactions; Coumarins - chemistry; cysteine; Cysteine - analysis; fluorescent dyes; Fluorescent Dyes - chemistry; glutathione; Glutathione - analysis; HeLa Cells; Humans; Maleimides - chemistry; metal ions; methionine; Microscopy, Fluorescence; Sulfonamides - chemistry

A fluorescent probe (N-(4-methyl-2-oxo-2H-chromen-7-yl)-2,4-dinitrobenzenesulfonamide), which exhibits high selectivity to glutathione and cysteine among amino acids including sulphur-containing methionine and metal ions, was synthesized. The experiments demonstrate that the fluorescent probe is a r...