D-penicillamine induces rat hepatic metallothionein

Intraperitoneal injection of D-Penicillamine (D-PA) at a dose range of 20-500 mg/kg increased rat hepatic but not renal and pancreatic metallothionein (MT). Elevated MT predominantly contained Zn. Maximal induction was obtained 18 h after a single injection of 200 mg D-PA/kg resulting in 148 +/- 18...

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Published inToxicology (Amsterdam) Vol. 42; no. 1; p. 23
Main Authors Heilmaier, H E, Jiang, J L, Greim, H, Schramel, P, Summer, K H
Format Journal Article
LanguageEnglish
Published Ireland 01.12.1986
Subjects
Animals
Copper - metabolism
Liver - drug effects
Male
Metallothionein - biosynthesis
Penicillamine - pharmacology
Rats
Rats, Inbred Strains
Time Factors
Zinc - metabolism
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Summary:Intraperitoneal injection of D-Penicillamine (D-PA) at a dose range of 20-500 mg/kg increased rat hepatic but not renal and pancreatic metallothionein (MT). Elevated MT predominantly contained Zn. Maximal induction was obtained 18 h after a single injection of 200 mg D-PA/kg resulting in 148 +/- 18 micrograms MT/g liver which was 16.4-times the control level of 9 +/- 2 micrograms MT/g. At 48 h after injection, MT declined to 18 +/- 9 micrograms MT/g liver. At maximal MT increase the content of total hepatic Zn but not of Cu was elevated. Increased amounts of Zn in liver homogenate, cytosol and MT could be detected approximately 4 h after injection of 200 mg D-PA/kg. Concomitantly there was a decrease in Zn bound to cytosolic non-MT ligands. All Zn changes reversed at 18 h. These data show that already single doses of D-PA cause induction of Zn-thionein in rat liver and lead to synchronous redistribution of Zn from endogenous sources to newly synthesized MT.
ISSN:0300-483X
1879-3185
DOI:10.1016/0300-483X(86)90089-2