EDMA 2000 as a matrix for high-performance liquid chromatography of human haemoglobin chains

An ethylenedimethacrylate polymer-based matrix (EDMA 2000) is described that shortens the time for separation of haemoglobin (Hb) chains to 30 min, compared with the use of C 4 large-pore Vydac columns, commonly used for reversed-phase HPLC of Hb variants. EDMA 2000 was successfully used for the iso...

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Bibliographic Details
Published inJournal of chromatography. B, Biomedical applications Vol. 656; no. 1; pp. 119 - 122
Main Authors Suttnar, Jiří, Fořtová, Hana, Brabec, Václav
Format Journal Article Conference Proceeding
LanguageEnglish
Published Amsterdam Elsevier B.V 03.06.1994
Elsevier
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Child
Chromatography, High Pressure Liquid
Erythrocytes - chemistry
Female
Fetal Blood - chemistry
Fundamental and applied biological sciences. Psychology
Heme - isolation & purification
Hemoglobins - isolation & purification
Hemoglobins, Abnormal - analysis
Hemoglobins, Abnormal - isolation & purification
Hemoproteins
Humans
Hydrogen-Ion Concentration
Indicators and Reagents
Infant
Metalloproteins
Methacrylates
Proteins
Spectrophotometry, Ultraviolet
Human
Separation method
Hemoprotein
Hemoglobin
Ethylene derivative polymer
Subunit
Structural analysis
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Summary:An ethylenedimethacrylate polymer-based matrix (EDMA 2000) is described that shortens the time for separation of haemoglobin (Hb) chains to 30 min, compared with the use of C 4 large-pore Vydac columns, commonly used for reversed-phase HPLC of Hb variants. EDMA 2000 was successfully used for the isolation of an abnormal β-chain of the unstable Hb recently characterized as Hb Nottingham.
ISSN:0378-4347
1572-6495
DOI:10.1016/0378-4347(94)00077-8