Effect of lipid physical state on the rate of peroxidation of liposomes

• Published in: Free radical biology & medicine Vol. 12; no. 2; pp. 113 - 119
• Main Authors: McLean, Larry R., Hagaman, Karen A.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 1992; Elsevier Science
• Subjects: Arachidonic acid; Arachidonic Acids - chemistry; Artificial membranes and reconstituted systems; Biological and medical sciences; Cholesterol - chemistry; Fluidity; Fluorescence polarization; Free Radicals; Fundamental and applied biological sciences. Psychology; Lipid Bilayers - chemistry; Lipid Peroxidation; Lipids - chemistry; Liposomes; Membrane Fluidity; Membrane physicochemistry; Molecular biophysics; Phase transitions; Phosphatidylcholines - chemistry; Temperature; Vesicles; Fluorescence polarization; Vesicles; Free radicals; Fluidity; Arachidonic acid; Phase transitions; Liposome; Phospholipid; Artificial membrane; Property composition relationship; Peroxidation
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/0891-5849(92)90004-Z

The effect of cholesterol on the rate of peroxidation of arachidonic acid and 1-palmitoyl-2-arachidonyl phosphatidylcholine (PAPC) in dimyristolphosphatidylcholine (DMPC) liposomes was examined above and below the phase transition temperature ( Tm) on the lipid. The rate of peroxidation of archidomic acid was more rapid below the phase transition temperature of the host lipid. At a temperature below the T m (4°C), increasing concentrations of cholesterol reduced the rate of peroxidation of arachidonic acid as judged by the production of thiobarbituric acid reactive substances. Above T m (37°C), cholesterol increased the rate of peroxidation of the fatty acid. Similarly, PAPC was peroxidized more rapidly at 4°C than at 37°C. However, cholesterol had little effect on the rate of peroxidation of PAPC at 4°C. The rate of peroxidation of arachidonic acid was related to the lipid bilayer fluidity as judged by fluorescence anisotropy measurements of diphenylhexatriene. The rate of peroxidation increased slowly with increasing rigidity of the probe environment when the bilayer was relatively fluid and more rapidly at the environment became more rigid. The increase in the rate of peroxidation of arachidonic acid in the less fluid host lipid was unrelated to differences in iron binding or to transfer of rachidonic acid to the aqueous phase. Decreasing the concentration of arachidoniac acid in DMPC to <2 mole% dramatically decreased the rate of peroxidation at 4°C, suggesting that formation of cluster of fatty acids at 4°C is required for rapid peroxidation. These data support the hypothesis that in an increase in the packing density of the acyl chains of peroxidazable lipids promotes lipid peroxidation in less fluid environments which favor phase separation.