Possible regulation of the cytosolic-free calcium concentration by Na+ spikes in immature cerebellar Purkinje cells

Isolated immature cerebellar Purkinje cell bodies often exhibit the spontaneous fluctuations of a cytosolic free Ca concentration ([ Ca]in), which was measured by fura-2 microfluorimetry. In silent cells, however, the increase of [Ca]in could be triggered by pharmacological depolarizations (adding v...

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Bibliographic Details
Published inNeuroscience letters Vol. 111; no. 3; p. 333
Main Authors Sorimachi, M, Morita, Y, Nakamura, H
Format Journal Article
LanguageEnglish
Published Ireland 06.04.1990
Subjects
Action Potentials - drug effects
Animals
Apamin - pharmacology
Calcium - analysis
Calcium - pharmacology
Calcium Channels - drug effects
Calcium Channels - physiology
Cytosol - physiology
Purkinje Cells - drug effects
Rats
Rats, Inbred Strains
Sodium - pharmacology
Sodium Channels - drug effects
Sodium Channels - physiology
Tetraethylammonium
Tetraethylammonium Compounds - pharmacology
Tetrodotoxin - pharmacology
Veratridine - pharmacology
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Summary:Isolated immature cerebellar Purkinje cell bodies often exhibit the spontaneous fluctuations of a cytosolic free Ca concentration ([ Ca]in), which was measured by fura-2 microfluorimetry. In silent cells, however, the increase of [Ca]in could be triggered by pharmacological depolarizations (adding veratridine or tetraethylammonium). In any case, these [Ca]in changes were sensitive to voltage-dependent channel blockers (tetrodotoxin and Ca channel blockers) and to an inhibitory transmitter (GABA). The results suggest that the spontaneous activation of voltage-dependent Na+ and Ca2+ channels might be involved in the increase of [Ca]in.
ISSN:0304-3940
DOI:10.1016/0304-3940(90)90284-G