A report on the demonstration of microfilariae of Brugia malayi in the brain of an experimental animal host, Mastomys natalensis

The brain tissues of microfilaraemic animals, Mastomys natalensis, which were earlier inoculated (s.c.) with Brugia malayi infective larvae (100 each) were examined for the occurrence of Mf. This was done by staining squash preparations of the brain tissues which were cleared off from the vascular p...

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Published inInternational journal for parasitology Vol. 25; no. 9; pp. 1123 - 1126
Main Authors Paily, K.P., Gomathi, K., Hoti, S.L., Balaraman, K., Dhanda, V.
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.09.1995
Elsevier Science
Subjects
Animals
Biological and medical sciences
Blood - parasitology
Brain - parasitology
Brain - pathology
brain tissue
Brugia malayi
Brugia malayi - isolation & purification
cryo-sections
Female
Filariasis - blood
Filariasis - pathology
Helminthic diseases
infection
Infectious diseases
Male
Mastomys natalensis
Medical sciences
microfilariae
Miscellaneous
Muridae - parasitology
Parasitic diseases
squash staining
microfilariae
infection
Brugia malayi
brain tissue
cryo-sections
Mastomys natalensis
squash staining
Animal model
Prevalence
Rodentia
Microfilaria
Parasite
Parasitosis
Helminthiasis
Infection
Vertebrata
Mammalia
Nematod disease
Infectivity
Helmintha
Cryosection
Nemathelminthia
Filariosis
Invertebrata
Nematoda
Brain (vertebrata)
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Summary:The brain tissues of microfilaraemic animals, Mastomys natalensis, which were earlier inoculated (s.c.) with Brugia malayi infective larvae (100 each) were examined for the occurrence of Mf. This was done by staining squash preparations of the brain tissues which were cleared off from the vascular piamater. Animals with blood Mf count of 50>/per 20 cu. mm were found to harbour Mf in the brain tissues. The Mf count in the brain varied from 5–86 81 cu. mm (sum of Mf detected in 3 tissue pieces, each of 27 cu. mm collected from 3 parts of the brain, viz., the cerebral hemispheres, cerebellum and medulla oblongata). The presence of Mf in the brain was confirmed by its detection in 20-μm-thick cryosections of the tissue. Also, fine needle aspirates of cerebral hemispheres of an animal showed live Mf.
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ISSN:0020-7519
1879-0135
DOI:10.1016/0020-7519(95)00030-6