Effects of platelet activating factor (PAF) and its receptor antagonist BN 52021 on isolated perfused guinea-pig heart

• Published in: Prostaglandins, leukotrienes and essential fatty acids Vol. 38; no. 3; pp. 189 - 194
• Main Authors: Viossat, I., Chapelat, M., Chabrier, P.E., Braquet, P.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.12.1989; Elsevier
• Subjects: Animals; Biological and medical sciences; Coronary Circulation - drug effects; Diterpenes; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; Ginkgolides; Guinea Pigs; Heart; Heart - drug effects; Heart Function Tests; Heart Rate - drug effects; In Vitro Techniques; L-Lactate Dehydrogenase - blood; Lactones - pharmacology; Myocardial Contraction - drug effects; Perfusion; Platelet Activating Factor - antagonists & inhibitors; Platelet Activating Factor - pharmacology; Vertebrates: cardiovascular system; Heart; Vertebrata; Mammalia; Guinea pig; Perfusion; Rodentia; Antagonist; Circulatory system; Arachidonic acid; Metabolism; Platelet activating factor; Isolated organ
• ISSN: 0952-3278; 1532-2823
• DOI: 10.1016/0952-3278(89)90071-9

The cardiac effects of PAF and its antagonist BN 52021 have been investigated on the isolated perfused guinea-pig heart maintained at a constant hydrostatic perfusion pressure of 80 cm water. In this model, PAF (1 × 10 −11 to 1 × 10 −7 moles) induced a dose-dependent coronary vasoconstriction, a decrease in heart rate and a fall in contractile force. BN 52021 (1 × 10 −6 to 2 × 10 −4 M) dose-dependently inhibited the vasospasm induced by PAF (1 × 10 −10 moles). BN 52021 also antagonized the decrease in coronary flow and heart rate, but not that of contractile force induced by a high dose of PAF (1 × 10 −7 moles). This dose of PAF also significantly (p < 0.001) provoked a marked release of TxB 2 but did not alter the generation of 6 Keto PGF 1α, PGE 2 or LTC 4. The PAF-induced increase in TxB 2 release was completely abolished by BN 52021.