Quantification of soluble serum HLA Class I antigens in healthy volunteers and AIDS patients

• Published in: Clinica chimica acta Vol. 174; no. 2; pp. 207 - 211
• Main Authors: Ferreira, A., Villar, M.L., Cermeño, J.C.Alvarez, Revilla, Y., Rodriguez, M.C.García, Fontán, G., González-Porqué, P.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 31.05.1988; Elsevier
• Subjects: Acquired Immunodeficiency Syndrome - blood; Acquired Immunodeficiency Syndrome - immunology; AIDS; AIDS/HIV; Biological and medical sciences; Blood Donors; ELISA; Enzyme-Linked Immunosorbent Assay; HLA Antigens - analysis; Humans; Immunodeficiencies; Immunodeficiencies. Immunoglobulinopathies; Immunopathology; Medical sciences; Monoclonal antibodies; Soluble HLA Class I antigens; Monoclonal antibodies; AIDS; Soluble HLA Class I antigens; ELISA; Infection; Human; Immunopathology; HLA-System; Immunological investigation; Viral disease; Major histocompatibility system; Serum; Hemopathy; ELISA assay
• ISSN: 0009-8981; 1873-3492
• DOI: 10.1016/0009-8981(88)90387-7

A solid-phase enzyme immunoassay (ELISA) has been used to quantify human soluble Class I histocompatibility antigens in serum samples from voluntary blood donors and AIDS patients. Statistical analysis of the results showed significantly raised levels ( p < 0.01) of free HLA Class I in sera from AIDS patients (2.95 ± 1.80 μg/ml) when compared with the blood donors (1.06 ± 0.6 μg/ml). The assay is specific, reproducible and easy to perform. Potential uses of this determination are discussed.