A novel derivative of the prosthetic group heme d1: S-methylporphyrindione d1

• Published in: Biochimica et biophysica acta Vol. 1116; no. 1; pp. 24 - 26
• Main Authors: Timkovich, Russell, Carlin, Richard T.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05.03.1992; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid; Cytochromes - chemistry; Fundamental and applied biological sciences. Psychology; Heme - analogs & derivatives; Heme - chemistry; Heme - isolation & purification; Heme d1; Heterocyclic compounds, pigments; Magnetic Resonance Spectroscopy; Mass specrometer; Mass Spectrometry; Mesoporphyrins - chemistry; Mesoporphyrins - isolation & purification; Nitrite Reductases; Other biological molecules; Suicide inactivation; Tetrapyrrolic pigments; Thiomethyl; Suicide inactivation; Thiomethyl; Mass specrometer; Heme d 1; Characterization; Prosthetic group; Heme; NMR spectrometry; Derived product; Mass spectrometry; Ultraviolet visible spectrometry
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(92)90123-C

Several derivatives of heme d 1 have been characterized by ultraviolet-visible, NMR, and mass spectrometry. Most arise from side reactions during the isolation of d 1 from the enzyme. One, however, has now been shown to correspond to the replacement of a meso proton by an S-methyl group. Since the prophyrin is not exposed to S-methyl-containing agents during its isolation, this raises hypotheses that it has its origin in vivo.