Specific molecular mass detection of endogenously released neuropeptides using in vivo microdialysis/mass spectrometry
The specific molecular detection of the endogenous neuropeptides methionine ([Met] 5) enkephalin and neurotensin released in vivo in rat brain has been accomplished using microdialysis and mass spectrometry. Microdialysis probes were implanted in specific brain regions and were used to collect sampl...
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Published in | Journal of neuroscience methods Vol. 62; no. 1; pp. 141 - 147 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
01.11.1995
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | The specific molecular detection of the endogenous neuropeptides methionine ([Met]
5) enkephalin and neurotensin released in vivo in rat brain has been accomplished using microdialysis and mass spectrometry. Microdialysis probes were implanted in specific brain regions and were used to collect samples from brain extracellular fluids in unanesthetized, freely moving animals. Microelectrospray/tandem mass spectrometry was used to achieve molecular-specific identification of the neuropeptides with a sensitivity in the amol/μl range. Measurements of the amounts of neuropeptides in the dialysates obtained from studies of KCl-stimulated release showed that [Met]
5-enkephalin from the globus pallidus/ventral pallidum region was present at a level of approximately 4–6 fmol/10 μl of dialysate and neurotensin from the hypothalamus of approximately 500 amol in 10 μl of dialysate. In this manuscript, we present the first data of a mass- and molecular-specific detection and quantitation of individual neuropeptides released in response to either intracerebrally or systemically administered compounds. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0165-0270 1872-678X |
DOI: | 10.1016/0165-0270(95)00070-4 |