Development of lipid A-imprinted polymer hydrogels that selectively recognize lipopolysaccharides

• Published in: Biosensors & bioelectronics Vol. 38; no. 1; pp. 215 - 219
• Main Authors: Ogawa, Kei-Ichi, Hyuga, Masumi, Okada, Tomoko, Minoura, Norihiko
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.10.2012; Elsevier
• Subjects: Adsorption; Adsorption isotherm; Binding Sites; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Gram-Negative Bacteria - isolation & purification; Hydrogels - chemistry; Lipid A; Lipid A - chemistry; Lipopolysaccharide; Lipopolysaccharides - isolation & purification; Lysine - analogs & derivatives; Molecular Imprinting; Molecular recognition; Octanes - chemistry; Phenylalanine - analogs & derivatives; Polymer hydrogel; Polymers - chemistry; Water Microbiology; Water Purification - methods; Lipopolysaccharide; Molecular recognition; Polymer hydrogel; Molecular imprinting; Lipid A; Adsorption isotherm; Lipids; Polymer; Hydrogel
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2012.05.028

To remove lipopolysaccharide (LPS) from pure water, we developed polymer hydrogels that selectively recognize LPS. A molecular imprinting technique was used to prepare the polymer hydrogels. We prepared the polymer hydrogels with LPS-binding sites by using acryloyllysine and acryloylphenylalanine as functional monomers and used lipid A as a template because it is the biologically active part of LPS and contains two phosphate groups. Co-existence of n-octane during the polymerization process was highly effective in promoting the formation of LPS-accessible sites on the surface of the hydrogels. Both an electrostatic and a hydrophobic interaction between the lipid A portion of LPS and the recognition site of the imprinted hydrogel are necessary for LPS recognition. The adsorption isotherm of LPS to the lipid A-imprinted hydrogels was Langmuir-type; the saturated adsorption capacity and the adsorption constant, calculated by applying an equation for Langmuir-type adsorption isotherms, were 1.0×10–11mol/cm2 and 2.5×105M−1, respectively. The imprinted hydrogels selectively recognized toxic LPS in a competition experiment in which two other kinds of LPS with similar chemical structures to that of the LPS of E. coli (toxic LPS) were adsorbed to the lipid A-imprinted hydrogels. ► We succeeded to prepare polymeric hydrogel with LPS-binding sites. ► The imprinted sites that LPS are accessible were formed by using n-octane. ► The imprinted hydrogels selectively recognized toxic LPS from a result. ► The adsorption isotherm of LPS to the lipid A-imprinted hydrogels was Langmuir-type.