A Mass Spectrometric Method for Use of Stable Isotopes as Tracers in Studies of Iron, Zinc, and Copper Absorption in Human Subjects

Stable isotopes of iron, zinc, and copper can be used singly and in combination as tracers of mineral metabolism in human subjects. A mass spectrometric method of analysis that uses chelation of these metals with tetraphenylporphyrin was developed. Absorption can be determined by measuring isotope a...

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Bibliographic Details
Published inThe Journal of nutrition Vol. 112; no. 7; pp. 1414 - 1424
Main Author Johnson, Phyllis E.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.07.1982
Subjects
Absorption
agricultural research
analysis
Copper
Copper - metabolism
Feces
Feces - analysis
human nutrition
Humans
Iron
Iron - metabolism
Iron Isotopes
Isotopes
Mass Spectrometry
Mass Spectrometry - methods
metabolism
Metalloporphyrins
methodology
methods
nutrition physiology
Zinc
Zinc - metabolism
Zinc Isotopes
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ISSN0022-3166
DOI10.1093/jn/112.7.1414

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Summary:Stable isotopes of iron, zinc, and copper can be used singly and in combination as tracers of mineral metabolism in human subjects. A mass spectrometric method of analysis that uses chelation of these metals with tetraphenylporphyrin was developed. Absorption can be determined by measuring isotope appearance in feces after a single pulse dose. The method is satisfactory for 54Fe, 67Zn, and 65Cu. Detection of 57Fe and 70Zn in feces is not very reliable. stable isotopes iron zinc copper metal absorption mass spectrometry
Bibliography:S20
A50
8234190
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ISSN:0022-3166
DOI:10.1093/jn/112.7.1414