Effects of berberine on the growth and immune performance in response to ammonia stress and high-fat dietary in blunt snout bream Megalobrama amblycephala

• Published in: Fish & shellfish immunology Vol. 55; pp. 165 - 172
• Main Authors: Chen, Qing-Qing, Liu, Wen-Bin, Zhou, Man, Dai, Yong-Jun, Xu, Chao, Tian, Hong-Yan, Xu, Wei-Na
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2016
• Subjects: Ammonia - toxicity; Ammonia stress; Animal Feed - analysis; Animals; Aquaculture; Berberine; Berberine - administration & dosage; Berberine - pharmacology; Cyprinidae - genetics; Cyprinidae - immunology; Cyprinidae - metabolism; Diet - veterinary; Diet, High-Fat - veterinary; Freshwater; Hepatocyte apoptosis; High-fat diet; Immunity; Megalobrama amblycephala; Oxidative stress; Oxidative Stress - drug effects; Random Allocation; Stress, Physiological - drug effects; Water Pollutants, Chemical - toxicity; Berberine; Oxidative stress; Ammonia stress; Hepatocyte apoptosis; Immunity; High-fat diet
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2016.05.023

This study aimed to figure out the effects of berberine on growth performance, immunity, oxidative stress and hepatocyte apoptosis of blunt snout bream (Megalobrama amblycephala) fed with high-fat diet. 320 fish (80.00 ± 0.90 g) were divided randomly into four trial groups (each with four replicates) and fed with 4 diets (normal diet, normal diet with 50 mg/kg berberine, high-fat diet, high-fat diet with 50 mg/kg berberine), respectively. At the end of the feeding trial, ammonia stress test was carried out for 5 days. The result showed the growth performance, immune parameters including plasm acid phosphatase (ACP) activities, lysozyme (LYZ) activities and alternative complement C3 and C4 contents were suppressed in fish fed with high-fat diets but improved in berberine diets compared with control (normal diet). Hepatopancreas oxidative status, the malondialdehyde (MDA), protein carbonyl (PC) and lipid peroxide (LPO) were increased significantly (P < 0.05) when fish were fed with high-fat diets. Berberine could slow the progression of the oxidative stress induced by high-fat through increasing superoxide dismutase (SOD) activities and total sulfydryl (T-SH) levels of fish. And the hepatocyte apoptosis in the high-fat group could also be alleviated by berberine. After the ammonia stress test, the accumulative mortality was extremely (P < 0.05) low in fish fed high-fat diet with berberine compared to other groups. It was concluded berberine as a functional feed additive significantly inhibited the progression of oxidative stress, reduced the apoptosis and enhanced the immunity of fish fed with high-fat diet. •Berberine could inhibite the progression of oxidative stress of fish fed with high-fat diet.•Berberine could reduce apoptosis of fish fed with high-fat diet.•Berberine could enhance the immunity of fish fed with high-fat diet.