MiR-31 modulates coelomocytes ROS production via targeting p105 in Vibrio splendidus challenged sea cucumber Apostichopus japonicus in vitro and in vivo

• Published in: Fish & shellfish immunology Vol. 45; no. 2; pp. 293 - 299
• Main Authors: Lu, Meng, Zhang, Pengjuan, Li, Chenghua, Zhang, Weiwei, Jin, Chunhua, Han, Qingxi
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2015
• Subjects: Animals; Apostichopus japonicus; Computational Biology; Gene Expression Regulation; Holothurioidea; Immunity, Innate; Lipopolysaccharides - pharmacology; Marine; MicroRNAs - genetics; MicroRNAs - metabolism; MiR-31; p105; Reactive Oxygen Species - metabolism; Respiratory burst; Stichopus - genetics; Stichopus - immunology; Stichopus - metabolism; Toll-Like Receptors - genetics; Toll-Like Receptors - metabolism; Vibrio; Vibrio - physiology; Vibrio splendidus; Respiratory burst; p105; Apostichopus japonicus; MiR-31
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2015.04.024

MiR-31 is a critical regulator of gene expression in many pathogenic processes in vertebrates. In this study, we identified p105 as a novel target of miR-31 in Apostichopus japonicus and investigated their regulatory roles in vitro and in vivo. The negative expression profiles between miR-31 and Ajp105 were detected in both LPS-exposed primary coelomocytes and Vibrio splendidus-challenged sea cucumber. Co-infection miR-31 mimics significantly depressed the expression of Ajp105 and increased ROS production in vitro. In contrast, miR-31 inhibitor significantly elevated the expression of Ajp105 and decreased ROS level. Consistently, miR-31 over-expression or Ajp105 silencing in vivo both greatly promoted ROS accumulation. Taken together, our findings confirmed that miR-31 could modulate respiratory burst via targeting Ajp105 during sea cucumber pathological development. •Ajp105 is firstly identified as one of putative targets of miR-31 in sea cucumber.•Their negative expression profiles were detected in vivo and in vitro.•MiR-31 could modulate Ajp105 expression and ROS production in vitro and in vivo.•Ajp105 silencing by siRNA also promoted respiratory burst in vitro and in vivo.