Tumor growth and adherence change the expression of macrophage Mac-2

• Published in: Cancer letters Vol. 69; no. 1; pp. 67 - 74
• Main Authors: Askew, David, Burger, Carol J., Elgert, Klaus D.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 15.04.1993; Elsevier
• Subjects: animal lectins; Animal tumors. Experimental tumors; Animals; Antigens, Differentiation - analysis; Biological and medical sciences; Cell Adhesion; Cell Division; cell-cycle kinetics; Experimental tumors, general aspects; Fibrosarcoma - pathology; Galectin 3; gene expression; Macrophages - chemistry; Macrophages - pathology; Male; Medical sciences; Methylcholanthrene; Mice; Mice, Inbred BALB C; Neoplasm Transplantation; RNA, Messenger - analysis; Tumors; cell-cycle kinetics; gene expression; animal lectins; Cell culture; Flow cytometry; Lectin; Membrane protein; Rodentia; Tumorigenicity; Gene expression; Adhesion; Carcinogenesis; In vitro; Northern blotting; Vertebrata; Mammalia; Mouse; Animal; Cell cycle; Tumor; Mechanism of action; Macrophage
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/0304-3835(93)90034-7

The galactose-specific animal lectin, Mac-2, has been identified in macrophage (MΦ) membrane, cytoplasmic, and nuclear fractions. Flow cytometric analyses showed that there is a decrease in membrane Mac-2 during tumor growth. After 24-h adherence there was an increase in the number of normal host (NH) and tumor-bearing host (TBH) Mac-2 + MΦ. Immunoblot analyses of NH and TBH MΦ identified changes in the subcellular localization of Mac-2. The increase in nuclear Mac-2 during tumor growth, and after prolonged adherence of NH and TBH MΦ, correlates with an increase in MΦ entering the late G 1 phases of the cell cycle. Northern blot analyses showed an increase in Mac-2 mRNA during tumor growth, and an increase in NH and TBH MΦ after 24-h adherence. Tumor growth is able to manipulate the immune system through MΦ by causing a down-regulation in membrane Mac-2 and an up-regulation in intracellular Mac-2. NH and TBH MΦ respond to adherence by expressing increased membrane and nuclear Mac-2, but TBH MΦ response is lower.