Differentiation-associated carbohydrate chain on human hematopoietic cells recognized by Clerodendron trichotomum lectin

• Published in: Leukemia research Vol. 11; no. 7; pp. 603 - 608
• Main Authors: Shichijo, Shigeki, Shibata, Hideki, Tsunosue, Rika, Shiotsuki, Kazuhide, Hara, Akinori, Ito, Katsumi, Shiraishi, Makoto, Yokoyama, Mitchel Mitsuo
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1987; Elsevier Science
• Subjects: aggregation; Biological and medical sciences; Carbohydrate Metabolism; Cell Differentiation - drug effects; Cell differentiation, maturation, development, hematopoiesis; Cell Line; Cell physiology; Clerodendron trichotomum; Erythrocytes - metabolism; flow cytometry; Fundamental and applied biological sciences. Psychology; Hematopoietic Stem Cells - metabolism; human hematopoietic cell; Humans; Lectin; Lectins - metabolism; Leukemia, Myeloid - pathology; Leukemia, Myeloid, Acute - pathology; Molecular and cellular biology; Neoplastic Stem Cells - metabolism; Plant Lectins; Receptors, Mitogen - metabolism; Tetradecanoylphorbol Acetate - pharmacology; Lectin; NANA; PNA; TPA; GalNH 3; aggregation; FITC; SBA; Fuc; human hematopoietic cell; flow cytometry; GlcNAc; cell line; HPLC; PBS; MoAb; GalNAc; BPA; Gal; Clerodendron trichotomum; GlcNH 3; EBv; CTL; Man; Glc; Hematopoietic cell; Cell differentiation
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/0145-2126(87)90032-4

In the screening of hematopoietic cell line cell aggregations, the extract of Clerodendron trichotomum seed was found to aggregate K-562 and KG-1 specifically. In the flow cytometric analysis using FITC-conjugated purified CTL, it was confirmed that CTL recognizes the specific carbohydrate(s) which seem to appear only in the early stages of differentiation of myeloid (KG-1) and erythroid (K-562) cell line cells and erythrocytes. The CTL binding to K-562 cells was decreased by TPA treatment which is known to induce rectrodifferentiation of K-562. It is also found that this carbohydrate(s) were shaded with NANA on the differentiated cells. In the erythrocyte, CTL receptor was partially shaded by NANA.