Dietary effects of a low-molecular weight fraction (<10 kDa) from shrimp waste hydrolysate on growth performance and immunity of rainbow trout (Oncorhynchus mykiss): Employing nanodelivery systems

• Published in: Fish & shellfish immunology Vol. 118; pp. 294 - 302
• Main Authors: Rashidian, Ghasem, Abedian Kenari, Abdolmohammad, Nikkhah, Maryam
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.11.2021
• Subjects: Animal Feed - analysis; Animals; aquaculture; blood serum; Chitosan; complement; Diet - veterinary; Dietary Supplements; fish; Fish Diseases - immunology; Fish Diseases - prevention & control; Growth performance; hydrolysates; immunity; interleukin-6; Liposomes; Low-molecular weight fraction; lysozyme; Molecular Weight; mucus; myeloperoxidase; Nanocapsules; nanocarriers; Nanoencapsulation; Nanoparticle Drug Delivery System; Oncorhynchus mykiss; Oncorhynchus mykiss - genetics; Oncorhynchus mykiss - growth & development; Oncorhynchus mykiss - immunology; Penaeidae - chemistry; proteinases; Rainbow trout; satiety; shellfish; shrimp; Shrimp wastes hydrolysate; Streptococcus iniae; Streptococcus iniae - immunology; survival rate; weight gain; Low-molecular weight fraction; Shrimp wastes hydrolysate; Streptococcus iniae; Nanoencapsulation; Growth performance; Rainbow trout
• ISSN: 1050-4648; 1095-9947; 1095-9947
• DOI: 10.1016/j.fsi.2021.09.014

Aquaculture by-products have been of great interest for producing protein hydrolysates with multiple biological activities. The present experiment was carried out to evaluate dietary effects of a low-molecular fraction (<10 kDa) from shrimp waste hydrolysate in forms of unprotected and nanocapsulated on growth and immunity of rainbow trout. Therefore, six diets were designed including a control diet (no supplementation), D1 (1 g kg−1 of unprotected fraction), D2 (1 g kg−1 chitosan nanocapsules), D3 (1 g kg−1 liposome nanocapsules), D4 (1 g kg−1 of fraction-loaded chitosan nanocapsules), D5 (1 g kg−1 of fraction-loaded liposome nanocapsules). Fish (0.91 ± 0.15 g) were fed with experimental diets until apparent satiation for six weeks followed by a 5-day experimental challenge with Streptococcus iniae. Results revealed that growth is strongly affected in fish receiving the fraction with D4 treatment showing the highest weight gain, SGR, final weight and the lowest FCR (p < 0.05). Nanocapsules without fraction did not show remarkable effects when compared to control group. In terms of serum and mucus immune parameters of lysozyme, complement activity, myeloperoxidase activity, and total protease, fish from D4 group showed the highest measured values followed by D5 (p < 0.05). Key immune related genes of IL-6 and TNF-α were noticeably up-regulated in fish from D1, D4, and D5 groups, which were consistent with survival rate after 5 days challenge with Streptococcus iniae. All together, the present findings highlighted the application of chitosan and liposome nanocarriers in aquaculture and potential of low-molecular weight fraction (<10 kDa) from shrimp wastes hydrolysate to improve growth performance and immune status of rainbow trout. •Chitosan can be used to encapsulate fractionated protein hydrolysate with 91% efficiency.•Nanocarriers loaded with fraction <10 kDa from shrimp waste significantly enhanced growth parameters of rainbow trout.•TNF-α and IL-6 were highly expressed in groups received fraction under 10 kDa in forms of unprotected and nanoencapsulated.•Fish fed with diets supplemented with fraction <10 kDa could significantly resist Streptococcus iniae infection.