Effects of oocytes exposure to bovine diarrhea viruses BVDV-1, BVDV-2 and Hobi-like virus on in vitro-produced bovine embryo development and viral infection

• Published in: Theriogenology Vol. 97; pp. 67 - 72
• Main Authors: da Silva Cardoso Pinto, V., Alves, M.F., de Souza Nunes Martins, M., Basso, A.C., Tannura, J.H., Pontes, J.H.F., Lima, M. Santos, Garcia da Silva, T., Okuda, L.H., Stefano, E., Romaldini, A.H.C.N., Arnold, D.R., Pituco, E.M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.07.2017
• Subjects: Animals; BVDV; Cattle; Diarrhea Virus 1, Bovine Viral - physiology; Diarrhea Virus 2, Bovine Viral - physiology; Embryo Culture Techniques - veterinary; Embryonic Development - physiology; Fertilization in Vitro - veterinary; IVP embryo development; Oocyte infection; Oocytes - physiology; Oocytes - virology; Pestivirus - physiology; Pestivirus Infections - embryology; Oocyte infection; BVDV; IVP embryo development
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2017.04.028

As production of in vitro (IVP) bovine embryos steadily increases, the sanitary risk associated with IVP embryos remains a concern. One of the greatest concerns is how BVDV may be transmitted through IVP embryos. The objective of this study was to evaluate the effects caused by BVDV-1, BVDV-2 and Hobi-like virus exposure during in vitro maturation on embryo development and viral infection. Abittior-derived oocytes were randomly assigned for in vitro maturation with serial concentrations of BVDV-1 (3.12 × 102 - 2.50 × 103 TCID50/100 μL), BVDV-2 (6.25 × 101 - 5.20 × 102 TCID50/100 μL) or Hobi-like virus (1.90 × 102 - 1.58 × 103 TCID50/100 μL) for 22–24 h. After maturation, oocytes were fertilized and embryo cultured following standard in vitro procedures. Embryo development was evaluated and percentage of respective, positive BVDV degenerated and viable embryos were evaluated by RT-qPCR. No concentration of BVDV-1 altered embryo development as measured by cleavage and blastocyst rates, compared to negative control group. However 100% of degenerated embryos and 50–100% of viable embryos tested positive for BVDV-1, depending on the viral concentration. BVDV-2 exposed oocytes had higher cleavage rates than the negative control group (60.2–64.1% vs 49.8%; P = 0.003–0.032). However, no difference was detected for blastocyst rates. In aadition, 100% of degenerated embryos and 20–50% of viable embryos tested positive for BVDV-2. Hobi-like virus treated oocytes had reduced cleavage rates for the three highest viral concentrations (33.3–38.0% vs 49.8% for negative controls; P ≤ 0.001–0.014). Blastocyst rates were only reduced in the 7.9 × 102 Hobi-like virus concentration (6.9 ± 0.9% vs 15.1 ± 1.6%; P = 0.009), when calculated by oocyte number. 50–80% of degenerated embryos tested positive for Hobi-like virus. No viable embryos from the Hobi-like virus treated oocytes tested positive. These results suggest that IVP embryos from BVDV-1 and -2 infected oocytes develop normally, but carry the virus. However, Hobi-like virus infected oocytes had reduced cleavage and cause pre-implantation embryo loss, but viable embryos did not carry the virus. •BVDV-1 during oocytes maturation had no effect on cleavage/blastocyst. RT-qPCR detected BVDV-1 in all degenerated embryos.•BVDV-2 during oocytes maturation had no effect on blastocyst. RT-qPCR detected BVDV-2 in 92-100% of degenerated embryos.•Hobi-like virus reduced cleavage and blastocyst rates. RT-qPCR detected Hobi-like virus in all degenerated embryos.•Washing viable embryos with trypsin (IETS recommendation) had no effect on removing BVDV-1, -2.