Oxygen consumption, ventilation frequency and cytochrome c oxidase activity in blue cod ( Parapercis colias) exposed to hydrogen sulphide or isoeugenol

• Published in: Comparative biochemistry and physiology. Toxicology & pharmacology Vol. 151; no. 1; pp. 57 - 65
• Main Authors: Forgan, Leonard G., Forster, Malcolm E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 2010
• Subjects: Anaesthesia; Anesthetics - pharmacology; Animals; Consciousness - drug effects; Cytochrome c oxidase; Electron Transport Complex IV - metabolism; Eugenol - analogs & derivatives; Eugenol - pharmacology; Female; Gills - drug effects; Gills - enzymology; Hydrogen Sulfide - pharmacology; Hydrogen sulphide; Isoeugenol; Male; Marine; Muscle, Skeletal - drug effects; Muscle, Skeletal - enzymology; Oxygen consumption; Oxygen Consumption - drug effects; Parapercis colias; Perciformes - metabolism; Pulmonary Ventilation - drug effects; Respiratory Mechanics - drug effects; Stupor - chemically induced; Teleostei; Time Factors; Ventilation frequency; Anaesthesia; Cytochrome c oxidase; Hydrogen sulphide; Isoeugenol; Oxygen consumption; Ventilation frequency
• ISSN: 1532-0456; 1878-1659
• DOI: 10.1016/j.cbpc.2009.08.008

The effects of hydrogen sulphide (H 2S) and isoeugenol exposure on activity, oxygen consumption (VO 2), ventilation frequency (Vf) and cytochrome c oxidase activity in a teleost fish are reported. In H 2S (200 µM Na 2S) exposed animals VO 2 and Vf decreased significantly (both to 40% of resting) after 30 min, concurrent with a loss of equilibrium and narcosis. Post-flushing, VO 2 increased to resting values, but Vf remained depressed ( P < 0.05) until 30 min of recovery. Subsequently, equilibrium and mobility were regained accompanied by increases in VO 2 (66%) and Vf (15%) between 60–70 min of recovery. Isoeugenol (0.011 g L − 1 ) exposed fish reached stage 4–5 of anaesthesia accompanied by decreases ( P < 0.05) in VO 2 (64%) and Vf (38%) by 35 min. Post-flushing, VO 2 and Vf recovered to resting values, followed by a rise ( P < 0.05) in VO 2 (45%) and Vf (25%). Overall, VO 2 in relation to the resting rate was reduced in isoeugenol treated animals. Conversely, VO 2 was increased ( P < 0.05) relative to the resting rate in H 2S exposed fish. 20 and 200 µM Na 2S reduced cytochrome c oxidase activity ( P < 0.05) in skeletal muscle and gill lamellae by between 69 and 97%, while isoeugenol had no effect in any tissue.