Effect of adrenalectomy and glucocorticoid treatment on the levels of an insulin-sensitive glycosyl-phosphatidylinositol in isolated rat hepatocytes

• Published in: Molecular and cellular endocrinology Vol. 68; no. 1; pp. R1 - R5
• Main Authors: Cabello, M.A., Sánchez-Arias, J.A., Liras, A., Mato, J.M., Felíu, J.E.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 02.01.1990; Elsevier
• Subjects: Adrenal Glands - physiology; Adrenalectomy; Animals; Biological and medical sciences; Dexamethasone - pharmacology; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Glucocorticoid; Glycolipids - metabolism; Glycosyl-phosphatidylinositol; Glycosylphosphatidylinositols; Insulin - pharmacology; Insulin resistance; Liver - drug effects; Liver - metabolism; Male; Medical sciences; Phosphatidylinositols - metabolism; Rat hepatocytes; Rats; Rats, Inbred Strains; Receptor, Insulin - drug effects; Receptor, Insulin - metabolism; Adrenalectomy; Insulin resistance; Rat hepatocytes; Glucocorticoid; Glycosyl-phosphatidylinositol; Endocrinopathy; Pancreatic hormone; Rat; Rodentia; In vitro; Insulin; Glycolipid; Protein hormone; Phosphatidylinositol; Target tissue resistance; Vertebrata; Experimental disease; Mammalia; Hepatocyte; Animal; Hormonal receptor
• ISSN: 0303-7207; 1872-8057
• DOI: 10.1016/0303-7207(90)90173-6

Insulin resistance caused by dexamethasone administration to rats was accompanied by a marked decrease in the hepatocyte content of an insulin-sensitive glycosyl-phosphatidylinositol, as well as by a blockade of its hydrolysis in response to this hormone. In contrast, bilateral adrenalectomy provoked a significant increase of the cellular glycosyl-phosphatidylinositol levels. Under all the assayed metabolic conditions, a close direct correlation was established between the basal content of this compound and the number of insulin receptors present in the isolated hepatocytes.