Heterogeneity of cellular expression of arachidonate 15-lipoxygenase: implications for biological activity

• Published in: Biochimica et biophysica acta Vol. 1003; no. 2; pp. 204 - 208
• Main Authors: Holtzman, M.J., Pentland, A., Baenziger, N.L., Hansbrough, J.R.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 08.06.1989; Elsevier; North-Holland
• Subjects: Analytical, structural and metabolic biochemistry; Arachidonate 15-Lipoxygenase - metabolism; Arachidonate Lipoxygenases - metabolism; Biological and medical sciences; Blood Cells - metabolism; Cells, Cultured; Chromatography, High Pressure Liquid; Cyclooxygenase; Enzymes and enzyme inhibitors; Epithelial Cells; Epithelium - metabolism; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Hydroxyeicosatetraenoic Acids - biosynthesis; Leukotriene; Lipoxygenase; Oxidoreductases; Prostaglandin; RP-HPLC; Leukotriene; Cyclooxygenase; Prostaglandin; PG; SP-HPLC; Lipoxygenase; HETE; Human; Enzyme; Leukocyte; Metabolite; Arachidonic acid derivatives; HPLC chromatography; Review; Biological activity; Eosinophil; Epithelial cell; Trachea; Arachidonate 15-lipoxygenase
• ISSN: 0005-2760; 0006-3002; 1879-145X; 1878-2434
• DOI: 10.1016/0005-2760(89)90257-9

Comparison of relative arachidonate 15-lipoxygenase activities in different types of human cell reveals striking differences in levels of product generation. Among available human cell types, the tracheal epithelial cell and the eosinophil have markedly higher levels of activity than other cell types with reported activity. Theories of functional significance for this enzymatic pathway will need to account for the selective expression of high levels of activity in a highly limited number of cell types.