In Vivo Measurement of Solute Transport Rates in a Bioartificial Organ

• Published in: Tissue engineering Vol. 5; no. 3; pp. 197 - 206
• Main Authors: Krohn, Julie A., Baker, Andrew R., Long, Jennifer L., Fournier, Ronald L., Byers, James P.
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.06.1999
• Subjects: Animals; Artificial Organs; Carbon Radioisotopes - pharmacokinetics; Glomerular Filtration Rate; Inulin - pharmacokinetics; Male; Models, Biological; Neovascularization, Physiologic; Permeability; Rats; Rats, Sprague-Dawley; Tissue Distribution
• ISSN: 1076-3279; 1557-8690
• DOI: 10.1089/ten.1999.5.197

A radioactive tracer technique was used to evaluate the in vivo mass transfer properties of a tissue engineered bioartificial organ. To obtain these measurements, bioartificial organs were first implanted in ten rats and allowed to vascularize for 4 weeks. After vascularization, radioactive inulin was placed within the cell chamber of the device. Following the addition of tracer, blood samples were taken over a 4-h time period and inulin levels were determined. The results of these experiments were interpreted using a compartmental model that describes the transport of inulin from the cell chamber, across the immunoisolation membrane, and into the neovascularized region contained within the adjacent scaffold material. Nonlinear regression analysis of the plasma inulin levels using a four-compartment pharmacokinetic model provided estimates of the membrane permeability, the product of the capillary wall surface area and capillary permeability, and the glomerular filtration rate (GFR). The permeability of the membrane was found to be 3.50 × 10 −5 ± 1.15 × 10 −5 cm/sec (95% confidence interval, n = 10), which compares favorably to previous in vitro permeability data for this membrane. The capillary wall permeability was found to be 0.0087 ± 0.0029 cm 3 /sec/100 g of tissue. This compares well to a reported value for inulin of 0.01 cm 3 /sec/100 g of tissue. The GFR was found to be 0.44 ± 0.07 ml/h/g BW, which compares well with a reported value of 0.40 µ /hr/g BW. The inulin tracer technique reported here is a useful tool for assessing the in vivo transport characteristics of a bioartificial organ as well as the vascularization within tissue engineered structures.