Determination of phosphoethanolamine in urine or in the presence of high taurine concentrations

• Published in: Analytical biochemistry Vol. 142; no. 2; pp. 511 - 514
• Main Authors: Gundlach, G., Käbisch, A., Voegeli, R., Pralle, H., Hartenfels, S.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.11.1984; Elsevier
• Subjects: 2-aminoethanolphosphate; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemical Precipitation; chromatographic determination; Chromatography, Ion Exchange; Ethanolamines - urine; Fundamental and applied biological sciences. Psychology; Humans; Hydrogen-Ion Concentration; Lead; lead-salt precipitation; Lipids; Other biological molecules; phosphoethanolamine; Solubility; Spectrometry, Fluorescence; taurine; Taurine - urine; taurine; chromatographic determination; lead-salt precipitation; phosphoethanolamine; 2-aminoethanolphosphate; Assay; Urine; Biological fluid; Phosphatidylethanolamine; Chemical precipitation; Clinical biology; Phospholipid; Ion exchange chromatography
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(84)90497-4

Phosphoethanolamine forms a low-solubility lead salt, which may be precipitated from urine together with inorganic phosphate, thus separating taurine, urea, and other interfering substances of chromatographic determination. Fluorometric detection of phosphoethanolamine with o-phthaldialdehyde after elution from a Dowex 50-X10 column enhances the specificity of the method once more. The method described is fast and easy to perform.