The identification of 5,6-dihydrouridine in normal human urine by combined gas chromatography/mass spectrometry
The identification of 5,6-dihydrouridine in normal human urine is reported. Partial purification and isolation of the compound by boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography preceded its characterization as a trimethylsilyl derivative by combined ga...
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Published in | Analytical biochemistry Vol. 181; no. 2; pp. 302 - 308 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
San Diego, CA
Elsevier Inc
01.09.1989
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | The identification of 5,6-dihydrouridine in normal human urine is reported. Partial purification and isolation of the compound by boronate gel affinity chromatography and reversed-phase high-performance liquid chromatography preceded its characterization as a trimethylsilyl derivative by combined gas chromatography/mass spectrometry. Structure proof is based upon a comparison of mass spectral and chromatographic features of the urinary component to that of an authentic reference sample. Additional data derived from high resolution mass measurements and deuterium isotopelabeling experiments provide confirmation of fragment ion structure. The poor detectability inherent in the
HPLC
uv
analysis of nucleosides is also discussed. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-2697 1096-0309 |
DOI: | 10.1016/0003-2697(89)90247-9 |