Blending Three Probiotics Alleviates Loperamide-Induced Constipation in Sprague-Dawley (SD)-Rats

• Published in: Food science of animal resources Vol. 44; no. 1; pp. 119 - 131
• Main Authors: Jang, Ye-Ji, Moon, Jin Seok, Kim, Ji Eun, Kim, Dayoung, Choi, Han Sol, Oh, Ikhoon
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Food Science of Animal Resources 01.01.2024; 한국축산식품학회
• Subjects: 축산학; constipation; BIOVITA 3 bacterial species; gut microbiota; probiotics
• ISSN: 2636-0772; 2636-0780
• DOI: 10.5851/kosfa.2023.e61

BIOVITA 3 bacterial species (BIOVITA 3), a probiotic blend powder containing IDCC 1301, IDCC 1201 and IDCC 1101, has been used as a food ingredient for gut health. However, its efficacy in improving constipation has not been reported. Therefore, we aimed to investigate the functional effects of oral administration of BIOVITA 3 as well as its component strains alone (at 1.0×10 CFU/day) in Sprague-Dawley (SD) rats with loperamide-induced constipation. The study included fecal analysis, gastrointestinal transit ratio, histopathological analysis, short chain fatty acids (SCFAs), and metagenome analysis. As results, the BIOVITA 3 group showed significant improvements in fecal number, water content, gastrointestinal transit ratio, and thickening of the mucosal layer. In the SCFAs analysis, all probiotic-treated groups showed an increase in total SCFAs compared to the loperamide-constipated group. Changes in microbial abundance and the diversity index of three groups (normal, constipated, and BIOVITA 3) were also defined. Of these, the BIOVITA 3 showed a significant improvement in loperamide-constipated SD-rats. This study suggests the possibility that BIOVITA 3 can be applied as an ingredient in functional foods to relieve constipation.