Cloning and 5′-flanking sequence of a rat cholesterol 7α-hydroxylase gene

• Published in: Biochimica et biophysica acta Vol. 1132; no. 3; pp. 337 - 339
• Main Authors: Chiang, John Y.L., Yang, T.P., Wang, D.P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20.10.1992; Elsevier
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Cholesterol 7-alpha-Hydroxylase - genetics; Cholesterol 7α-hydroxylase; Cloning, Molecular; DNA; Fundamental and applied biological sciences. Psychology; Gene cloning; Gene sequence; Genes. Genome; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Promoter Regions, Genetic; Rats; Regulatory element; Restriction Mapping; Gene cloning; Cholesterol 7α-hydroxylase; Gene sequence; Regulatory element; Vertebrata; Mammalia; Gene; Rat; Nucleotide sequence; Enzyme; Transcription promoter; Rodentia; Restriction map; Regulatory sequence; Flanking sequence; Cholesterol 7α-monooxygenase
• ISSN: 0167-4781; 0006-3002; 1879-2634
• DOI: 10.1016/0167-4781(92)90175-Y

A cholesterol 7α-hydroxylase gene containing 8 kb of the 5′-flanking region and 5 kb of the transcription region which covers exons 1 to 5 was isolated from a rat genomic library. The 2015 bp nucleotide sequence 5′-upstream from the start codon was determined. This promotor region contains many liver-enriched or -specific elements (TGT3, HNF/LF-B1), putative hormone responsive elements (TRE, GRE, RRE or RARE) and ubiquitous transcription factor binding sites (NF-1, OCT-1). In addition, 21 CA repeats which have potential to form the Z-DNA structure were found in this region. These putative regulatory elements and repetitive motifs may play roles in the regulation of cholesterol 7α-hydroxylase gene in the liver. The sequence identity of the rat gene to the human gene in this region is low. Only liver-enriched elements are conserved in the cholesterol 7α-hydroxylase genes.