Receptor-mediated internalization of angiotensin II in bovine adrenal medullary chromaffin cells in primary culture

• Published in: Regulatory peptides Vol. 53; no. 1; pp. 77 - 86
• Main Authors: Wang, Jun Ming, Llona, Isabel, De Potter, Werner P.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 31.08.1994; Amsterdam Elsevier
• Subjects: Adrenal Medulla - drug effects; Adrenal Medulla - metabolism; Angiotensin II - metabolism; Angiotensin II - pharmacology; Animals; Arsenicals - pharmacology; Binding; Biological and medical sciences; Cattle; Cell receptors; Cell structures and functions; Cells, Cultured; Endocytosis; Endocytosis - drug effects; Fundamental and applied biological sciences. Psychology; Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors; Iodine Radioisotopes; Kinetics; Molecular and cellular biology; Monensin - pharmacology; Peptide; Receptors, Angiotensin - physiology; Saralasin - pharmacology; Binding; Peptide; Endocytosis; Adrenal medulla; Ox; Binding site; Chromaffin cell; Renin angiotensin system; Vertebrata; Mammalia; Internalization; Artiodactyla; Angiotensin II; Ungulata; Hormonal receptor
• ISSN: 0167-0115; 1873-1686
• DOI: 10.1016/0167-0115(94)90161-9

Binding and internalization of angiotensin II (AII) were studied on bovine adrenal medullary cells in primary culture. Binding of [ 125I]AII was reversible, saturable, specific and showed high affinity. AII was found to be internalized by bovine adrenal medullary cells. Monensin increased whereas phenylarsine oxide (PhAsO) decreased the internalization. Excess of unlabelled AII or saralasin could block the internalization, indicating a receptor mediated internalization process. The kinetic analysis indicated that, during the first 4 min, about 25% of the membrane bound ligand was internalized per min and the recycling of internalized ligand and receptor initiated around 4 min.