A method for concentrating dilute solutions of macromolecules

• Published in: Analytical biochemistry Vol. 133; no. 1; pp. 260 - 263
• Main Authors: Vartak, H.G., Rele, M.V., Rao, M., Deshpande, V.V.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.08.1983; Elsevier
• Subjects: Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; Biological and medical sciences; carbohydrate concentration; Carbohydrates - analysis; Cellulase - isolation & purification; concentration of macromolecules; dewatering of macromolecules; DNA concentration; Fundamental and applied biological sciences. Psychology; Glucose - isolation & purification; Hydrogen-Ion Concentration; Macromolecular Substances; Molecular Weight; Nucleic Acids - analysis; polyacrylate gel; protein concentration; Proteins - analysis; protein concentration; dewatering of macromolecules; DNA concentration; polyacrylate gel; concentration of macromolecules; carbohydrate concentration; Biological macromolecule; Concentration; Technique; Acrylate polymer; Dilute solution
• ISSN: 0003-2697; 1096-0309
• DOI: 10.1016/0003-2697(83)90252-X

A simple, inexpensive acrylate polymer which has a capacity to absorb 170 ml of water per g has been developed. It can be used to concentrate dilute solutions of macromolecules such as proteins, nucleic acids, and carbohydrates. The polymer absorbs only low-molecular-weight substances such as glucose, sucrose, and inorganic salts. It can replace the various conventional concentration methods. No special device or electricity is needed for the concentration. The inexpensive polymer, molded in the form of rods, can be very conveniently used as “disposable concentration sticks.”