Genetic and physical analysis of the ilvBC-leu region in Bacillus subtilis

We describe the cloning of a 6.0-kb PstIfragment of the Bacillus subtilis genome which contains much of the ilvBC-leu gene cluster. This plasmid clone and two others that had been previously isolated were characterized physically and genetically to permit the construction of a physical map of this r...

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Bibliographic Details
Published inGene Vol. 32; no. 1; pp. 49 - 56
Main Authors Mackey, C.J., Warburg, R.J., Halvorson, H.O., Zahler, S.A.
Format Journal Article
LanguageEnglish
Published Lausanne Elsevier B.V 01.12.1984
Amsterdam Elsevier
New York, NY
Subjects
Bacillus subtilis - drug effects
Bacillus subtilis - genetics
Bacteriology
Biological and medical sciences
DNA, Bacterial - genetics
DNA, Recombinant
Drug Resistance, Microbial
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Leucine - analogs & derivatives
Leucine - pharmacology
Microbiology
Morphology, structure, chemical composition
plasmid vector
Plasmids
Recombinant DNA
Transduction, Genetic
Transformation, Bacterial
λphage
plasmid vector
Km
SSC
SDS
Cm
Recombinant DNA
R(superscript)
bp
Ap
Tc
λphage
Δ
LB
kb
Genetic mapping
Bactériophage lambda
Bacillus subtilis
Bacillaceae
Virus
Lambda phage group
Bacillales
Styloviridae
Restriction map
Bacteria
Bactériophage
Molecular cloning
Vector
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Summary:We describe the cloning of a 6.0-kb PstIfragment of the Bacillus subtilis genome which contains much of the ilvBC-leu gene cluster. This plasmid clone and two others that had been previously isolated were characterized physically and genetically to permit the construction of a physical map of this region that is correlated to the genetic map.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(84)90031-3