Molecular screening of Anaplasmataceae in ticks collected from cattle in Corsica, France

• Published in: Experimental & applied acarology Vol. 81; no. 4; pp. 561 - 574
• Main Authors: Cicculli, Vincent, DeCarreaux, Dorine, Ayhan, Nazli, Casabianca, François, de Lamballerie, Xavier, Charrel, Remi, Falchi, Alessandra
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.08.2020; Springer Nature B.V; Springer Verlag
• Subjects: Anaplasmataceae; Animal Ecology; Animal Genetics and Genomics; Animal Systematics/Taxonomy/Biogeography; Animals; Arachnids; Bacteria; Biomedical and Life Sciences; Cattle; Confidence intervals; Deoxyribonucleic acid; DNA; Domestic animals; Economic impact; Entomology; Epidemiology; Gene sequencing; Ixodidae; Life Sciences; Maximum likelihood estimation; New species; Pathogenesis; Phylogeny; Polymerase chain reaction; Public health; RpoB protein; rRNA; rRNA 23S; Ticks; Corsica; Anaplasmataceae; Cattle; Corsica; Ticks; Anaplasma; Ehrlichia
• ISSN: 0168-8162; 1572-9702
• DOI: 10.1007/s10493-020-00527-w

Bacteria belonging to the family Anaplasmataceae cause infections in humans and domestic animals. The consequences of infection can be significant economic losses for farmers. To better understand the epidemiology of tick-borne Anaplasmataceae in Corsica, we used molecular methods to detect and characterize Anaplasmataceae in ixodid ticks collected from cattle. Anaplasmataceae were detected by using a real-time polymerase chain reaction (PCR) targeting the 23S rRNA gene. Partial sequencing of rpoB and g roEL allowed identifying species and conducting phylogenetic analyses. Infection rates were calculated using maximum likelihood estimation (MLE) with 95% confidence intervals (CIs). In total, 597 Rhipicephalus bursa , 216 Hyalomma marginatum , and seven Ixodes ricinus were collected from cattle during July–August 2017 and July–December 2018. Overall, Anaplasmataceae DNA was detected in 15 of 255 tick pools (MLE = 1.7%; 95% CI 0.9–2.7%). The molecular analysis revealed two species within the genus Anaplasma : A. marginale and A. phagocytophilum . We also detected bacteria within the genus Ehrlichia : we confirmed the detection of E. minasensis DNA in H. marginatum and R. bursa tick pools collected from cattle in Corsica and detected, for the first time to our knowledge, Candidatus E. urmitei in Corsican R. bursa ticks and a potential new species, Candidatus E. corsicanum. Further studies are needed to ascertain the pathogenesis and zoonotic potential of the strains and their importance for animals and public health.