Isolation and chemical characterization of algal polysaccharides from the green seaweed Ulva clathrata (Roth) C. Agardh
In order to obtain information on the content and composition of the water-soluble polysaccharides from Ulva clathrata , an extraction at 60°C, in different media, was performed: water, EDTA and HCl (F-I), each followed by a sequential extraction in NaOH 0.1 M (F-II). The extracts were recovered and...
Saved in:
Published in | Journal of applied phycology Vol. 23; no. 3; pp. 537 - 542 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.06.2011
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | In order to obtain information on the content and composition of the water-soluble polysaccharides from
Ulva clathrata
, an extraction at 60°C, in different media, was performed: water, EDTA and HCl (F-I), each followed by a sequential extraction in NaOH 0.1 M (F-II). The extracts were recovered and analyzed for total carbohydrates, proteins, rhamnose, uronic acids and sulfate content. Differences were obtained in the yield and composition in both fractions of the different media (F-I and F-II). Higher yields resulted in the first fraction on all media. HCl extraction was the best in both fractions (14.83 ± 1.5% and 5.96 ± 1.1%, F-I and F-II, respectively). In all cases, F-I was more sulfated ranging from 27.87% to 35.8% and higher in rhamnose content, whereas F-II had higher protein and slightly higher uronic acid content. FTIR spectra showed that soluble polysaccharides from the green seaweed
U. clathrata
are sulfated polysaccharides, similar to ulvan obtained from other
Ulva
species and confirmed by the
1
H-NMR spectrum, where the characteristic signal for the deoxy sugar (rhamnose) is present. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0921-8971 1573-5176 |
DOI: | 10.1007/s10811-010-9629-0 |