Determination of chloramphenicol in muscle, liver, kidney and urine of pigs by means of immunoaffinity chromatography and gas chromatography with electron-capture detection

• Published in: Journal of chromatography. B, Biomedical applications Vol. 673; no. 2; pp. 197 - 204
• Main Authors: Gude, Th, Preiss, A., Rubach, K.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 17.11.1995
• Subjects: Animals; Anti-Bacterial Agents - analysis; Anti-Bacterial Agents - urine; Antibodies; Chloramphenicol - analysis; Chloramphenicol - urine; Chromatography, Affinity - methods; Chromatography, Affinity - statistics & numerical data; Chromatography, Gas - methods; Chromatography, Gas - statistics & numerical data; Indicators and Reagents; Kidney - chemistry; Liver - chemistry; Mathematics; Microchemistry; Muscles - chemistry; Sensitivity and Specificity; Swine
• ISSN: 0378-4347; 1572-6495
• DOI: 10.1016/0378-4347(95)00280-5

A rapid and specific clean-up procedure based on immunoaffinity chromatography (IAC) with polyclonal antibodies for the gas chromatographic determination with electron-capture detection of chloramphenicol in pig muscle tissue, organs and urine is described. A commercially available IAC material was used for the analysis. A decrease in the capacity of the column after being used more than 100 times was observed. Mean recoveries were 69, 54, 62 and 95% for spiked pig muscle tissue, liver, kidney and urine, respectively. The limit of detection was 0.2 μg/kg for muscle tissue, 2.0 μg/kg for liver and kidney and 0.4 μg/kg for urine.