The role of active immunization against inhibin α-subunit on testicular development, testosterone concentration and relevant genes expressions in testis, hypothalamus and pituitary glands in Yangzhou goose ganders

• Published in: Theriogenology Vol. 128; pp. 122 - 132
• Main Authors: Akhtar, Muhammad Faheem, Wei, Quanwei, Zhu, Huanxi, Chen, Zhe, Ahmad, Ejaz, Zhendan, Shi, Shi, Fangxiong
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2019
• Subjects: Animals; Anti-Mullerian Hormone - metabolism; Cholesterol Side-Chain Cleavage Enzyme - metabolism; Geese - growth & development; Geese - immunology; Geese - metabolism; Gene Expression; Gonadotropin-Releasing Hormone - metabolism; Hypothalamus - metabolism; Inhibin immunization; Inhibins - immunology; Luteinizing Hormone - metabolism; Male; Pituitary Gland - metabolism; Spermatogenesis; Testis; Testis - growth & development; Testis - immunology; Testis - metabolism; Testosterone - blood; Vaccination - veterinary; Yangzhou goose; Testis; Yangzhou goose; Inhibin immunization; Gene expression
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2019.01.039

The present study was designed to investigate the potential role of immunization against inhibin on testicular development, plasma testosterone concentration and expression of relevant genes in hypothalamus, pituitary, Leydig and Sertoli cells in Yangzhou ganders. For this purpose, Yangzhou ganders, n = 30 were divided into groups A and B. Group B ganders were actively immunized against inhibin α-subunit, while group A ganders were immunized with bovine serum albumin (BSA), which served as control. Immunization against inhibin elevated testes weights. In addition, immunization against inhibin elevated GnRH, StAR, CYP11A1 and AMH mRNA transcription expressions as depicted by qRT-PCR. Furthermore, hypothalamic GnRH-I mRNA expressions were up regulated, while GnIH mRNA transcription expression showed reciprocal expression on day 227. LH-β mRNA transcription expression remained unaffected. In conclusion, our findings suggest that active immunization against inhibin affect spermatogenesis and testicular development through regulations of hypothalamic, pituitary and testicular genes expressions. •Effect of immunization against inhibin on adult Yangzhou ganders was quantified.•Immunization against inhibin α subunit effected Sertoli cell development.•Inhibin immunization is involved in testosterone regulation in Yangzhou ganders.