Partial purification of Chironex fleckeri (sea wasp) venom by immunochromatography with antivenom

Chironex fleckeri crude venom was partially purified using immobilized commercially available ovoid antivenom. The antibody preparation reacted with lethal, hemolytic, dermonecrotic and mouse writhing (pain) factors in the crude venom. The lethal activity was purified five fold, while the specific e...

Full description

Saved in:
Bibliographic Details
Published inToxicon (Oxford) Vol. 24; no. 4; p. 416
Main Authors Calton, G J, Burnett, J W
Format Journal Article
LanguageEnglish
Published England 1986
Subjects
Animals
Antivenins - pharmacology
Chick Embryo
Chromatography, Affinity
Chromatography, Agarose
Cnidaria
Cnidarian Venoms - antagonists & inhibitors
Cnidarian Venoms - isolation & purification
Cnidarian Venoms - toxicity
Electrophoresis, Polyacrylamide Gel
In Vitro Techniques
Mice
Molecular Weight
Rats
Scyphozoa
Online AccessGet more information

Cover

More Information
Summary:Chironex fleckeri crude venom was partially purified using immobilized commercially available ovoid antivenom. The antibody preparation reacted with lethal, hemolytic, dermonecrotic and mouse writhing (pain) factors in the crude venom. The lethal activity was purified five fold, while the specific eluate contained lower quantities of hemolytic, dermonecrotic and mouse writhing activities than did the crude venom.
ISSN:0041-0101
DOI:10.1016/0041-0101(86)90202-3