The serum capacity to solubilize immune complexes (ICSC) measured by an enzyme-anti-enzyme complex probe

The capacity to solubilize immune complexes can be readily measured by incubating the test serum with a suspension of an immune precipitate formed by beta-galactosidase and anti-beta-galactosidase antibody, and then reading the enzyme units (EU) liberated in the clear supernatant. Our method is rapi...

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Bibliographic Details
Published inJournal of immunological methods Vol. 77; no. 1; p. 119
Main Authors Migliorini, P, Chieregatti, G, Trovatello, G, Cantarella, S, Fenoglio, D, Celada, F
Format Journal Article
LanguageEnglish
Published Netherlands 28.02.1985
Subjects
Antigen-Antibody Complex
beta-Galactosidase - immunology
Blood
Complement Pathway, Alternative
Humans
Immunoenzyme Techniques
Kinetics
Solubility
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Summary:The capacity to solubilize immune complexes can be readily measured by incubating the test serum with a suspension of an immune precipitate formed by beta-galactosidase and anti-beta-galactosidase antibody, and then reading the enzyme units (EU) liberated in the clear supernatant. Our method is rapid and inexpensive; it can be performed in plates and read in scanning colorimeters. Although on large numbers of observations the ICSC is significantly correlated with the CH50, a few discordant cases suggest that solubilization and haemolysis are functions of the alternative and classical pathways of complement respectively.
ISSN:0022-1759
DOI:10.1016/0022-1759(85)90189-9