A distal region enhances the prolactin induced promoter activity of the rabbit αs1-casein gene

• Published in: Molecular and cellular endocrinology Vol. 87; no. 1; pp. 147 - 156
• Main Authors: Pierre, S., Jolivet, G., Devinoy, E., Théron, M.C., Maliénou-N'Gassa, R., Puissant, C., Houdebine, L.M.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.09.1992; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Caseins - biosynthesis; Caseins - genetics; Cells, Cultured; Chloramphenicol acetyl transferase; CHO Cells; Cricetinae; Enhancer Elements, Genetic; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Genes, Synthetic; HC11 cells; Mammary Glands, Animal - cytology; Miscellaneous; Organoids; Pregnancy; Prolactin - pharmacology; Promoter Regions, Genetic - drug effects; Proteins; Rabbits - genetics; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - genetics; Transfection; Transient expression; Transfection; Organoids; CHO cells; HC11 cells; Chloramphenicol acetyl transferase; Transient expression; Vertebrata; Mammalia; Casein; Adenohypophyseal hormone; Phosphoproteins; Prolactin; Transcription promoter; Hormonal regulation; Rabbit; Regulatory sequence; Lagomorpha; Gene expression
• ISSN: 0303-7207; 1872-8057
• DOI: 10.1016/0303-7207(92)90243-Y

Casein gene expression is induced in the rabbit mammary gland by prolactin (PRL). αs1-casein is the major casein secreted into milk. In order to define the position of the DNA sequences involved in the control of rabbit αs1-casein gene regulation by PRL, chimeric genes were constructed between upstream regions of the rabbit αs1-casein gene and the chloramphenicol acetyl transferase (CAT) reporter gene. A series of 5'-deleted fusion genes was obtained by nuclease digestion of the αs1-casein gene upstream region. These gene constructs were transfected into rabbit primary mammary cells, or cotransfected in CHO cells with the plasmid coding for the rabbit mammary receptor (PRL-R). A regulatory region has been located between nt −3768 and −3155. This region enhances the prolactin induced promoter activity of the αs1-casein gene. It might possess or cooperate with prolactin responsive elements located further downstream in the αs1-casein gene.