Molecular cloning and in situ hybridization histochemistry for rat μ-opioid receptor
We cloned a cDNA for the rat μ-opioid receptor from a rat thalamus cDNA library. The deduced amino-acid sequence of rat μ-opioid receptor consists of 398 residues with the features shared by the members of the G-protein coupled receptor family, and is 59% and 60% identical with those of rat κ-opioid...
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Published in | Neuroscience research Vol. 18; no. 4; pp. 315 - 322 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier Ireland Ltd
1994
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | We cloned a cDNA for the rat μ-opioid receptor from a rat thalamus cDNA library. The deduced amino-acid sequence of rat μ-opioid receptor consists of 398 residues with the features shared by the members of the G-protein coupled receptor family, and is 59% and 60% identical with those of rat κ-opioid and mouse δ-opioid receptors, respectively. Northern blot analysis showed that expression of μ-opioid receptor mRNA was intensive in the thalamus, striatum, hypothalamus and pons-medulla, moderate in the hippocampus and midbrain, and slight in the cerebral cortex and cerebellum. More detailed distribution of the mRNA in the rat brain was examined using the in situ hybridization technique. Intense expression of μ-opioid receptor mRNA was observed in the internal granular and glomerular layers of the olfactory bulb, caudate putamen, nucleus accumbens, medial raphe nucleus, inferior colliculus, parabrachial nucleus, locus coeruleus, nucleus solitary tract and ambiguus nucleus. Furthermore, μ-opioid receptor mRNA was moderately expressed in the hippocampus, globus pallidus, ventral pallidus, arcuate hypothalamic nucleus, supramammillary nucleus, superior colliculus, periacqueductal gray, and several nuclei of lower brain stem, including raphe magnus nucleus, reticular gigantocellular nucleus and lateral paragigantocellular nucleus. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0168-0102 1872-8111 |
DOI: | 10.1016/0168-0102(94)90167-8 |