Slide immunoenzymatic assay for human IgE (SIA-IgE)

A rapid and inexpensive method is described for determination of human serum IgE using 5-microliter samples. The slide immunoenzymatic assay for IgE (SIA-IgE) is carried out on a glass slide in which up to 24 samples can be tested including controls and standards; up to four of these slides can be r...

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Bibliographic Details
Published inJournal of immunological methods Vol. 90; no. 1; p. 137
Main Authors Conway de Macario, E, Macario, A J, Jovell, R J
Format Journal Article
LanguageEnglish
Published Netherlands 10.06.1986
Subjects
Antibodies, Anti-Idiotypic
Antibody Specificity
Colorimetry
Cross Reactions
Humans
Immunoenzyme Techniques - instrumentation
Immunoglobulin E - analysis
Immunoglobulin E - immunology
Radioimmunosorbent Test
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Summary:A rapid and inexpensive method is described for determination of human serum IgE using 5-microliter samples. The slide immunoenzymatic assay for IgE (SIA-IgE) is carried out on a glass slide in which up to 24 samples can be tested including controls and standards; up to four of these slides can be read automatically in conventional vertical beam readers. Flat circles on the slide are covered with a layer of biotinylated antibody specific for human IgE (trapping antibody). Five microliters of serum sample is dropped to cover each circle, and the slide is incubated. The circles are washed with water, dried, incubated under 10 microliters of enzyme-labeled antibody to human IgE, then washed again, and covered with 10 microliters of enzyme substrate. The intensity of color generated is measured at the proper wavelength. The method is simple, accurate and non-radioactive and can be completed within 2 h.
ISSN:0022-1759
DOI:10.1016/0022-1759(86)90394-7