Mitogenicity of the earthworm's ( eisenia foetida) insulin-like proteins

• Published in: Comparative biochemistry and physiology. B, Comparative biochemistry Vol. 104; no. 4; pp. 723 - 729
• Main Authors: Hrẑenjak, Maja, Kobrehel, Đorđe, Levanat, Sonja, Jurin, Mislav, Hrẑenjak, Terezija
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.04.1993; Elsevier Science
• Subjects: Animals; Annelida and closely related phyla: sipuncula. Echiura. Nemertinea; Biological and medical sciences; Cell Division - drug effects; Chromatography, Gel; Cricetinae; Electrophoresis, Polyacrylamide Gel; Female; Fibrosarcoma; Fundamental and applied biological sciences. Psychology; Glycoproteins - isolation & purification; Humans; Immunoblotting; Insulin - isolation & purification; Insulin - pharmacology; Invertebrates; Lipoproteins - isolation & purification; Male; Melanoma, Experimental; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Mitogens; Oligochaeta; Radioimmunoassay; Tumor Cells, Cultured; Uterine Cervical Neoplasms; Cell proliferation; Glycoproteins; Insulin; Annelida; Oligochaeta; Biological activity; Vertebrata; Mammalia; Analog; Mitogen; Eisenia fetida; Invertebrata; Tumor cell
• ISSN: 0305-0491
• DOI: 10.1016/0305-0491(93)90204-I

1. 1. Biologically active glycolipoprotein complex (G-90), isolated from whole earthworm tissue extract ( Eisenia foetida), was separated into seven fractions by gel-filtration. 2. 2. It has been shown by radioimmunoassay that each of the fractions, except the lightest one, is cross-reactive with porcine anti-insulin antibodies. Molecules that possess such activity were detected by immunoblotting. 3. 3. All fractions, except the heaviest and the lightest one, stimulate mammalian normal and transformed cell proliferation in serum-free conditions in vitro. The intensity of stimulation depends on cell type. Stimulation is completely abolished if the medium is supplemented with fetal calf serum.