Hormonal control of collagenase inhibitor production in rabbit uterine cervical fibroblast-like cells

• Published in: Biochimica et biophysica acta Vol. 883; no. 3; pp. 517 - 522
• Main Authors: SAKYO, K, ITO, A, OGAWA, C, MORI, Y
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.10.1986; Elsevier; North-Holland
• Subjects: 17β-Estradiol; Animals; Biological and medical sciences; Cells, Cultured; Cervix Uteri - metabolism; Chromatography - methods; Collagenase inhibitor; Enzyme Inhibitors - biosynthesis; Estradiol - pharmacology; Female; Fibroblasts - metabolism; Fundamental and applied biological sciences. Psychology; Hormonal regulation; Hormone metabolism and regulation; Mammalian female genital system; Microbial Collagenase - antagonists & inhibitors; Progesterone; Progesterone - pharmacology; Rabbit uterus; Rabbits; Tissue Inhibitor of Metalloproteinases; Vertebrates: reproduction; 17β-Estradiol; Progesterone; Rabbit uterus; APMA; Collagenase inhibitor; Hormonal regulation; Vertebrata; Mammalia; Female genital system; Enzyme inhibitor; Rabbit; Collagenase; Uterine cervix; Lagomorpha; Fibroblast; Estradiol
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(86)90292-8

Rabbit uterine cervical fibroblast-like cells maintained in fetal calf serum-free medium were found to biosynthesize and secrete a collagenase inhibitor into the culture medium. All the properties of this inhibitor were similar to those that have been described so far for the tissue inhibitor of metalloproteinases. Both progesterone and 17β-estradiol significantly increased the level of collagenase inhibitor without the proliferation of cells. These data suggest that both progesterone and estradiol regulate collagenolysis in the uterus bifunctionally by acceleration of the inhibitor production in addition to their known inhibitory actions towards collagenase biosynthesis.